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2009-Diffusion-Lab-Group--5-C

Page history last edited by Madi Taylor 15 years, 1 month ago

Diffusion Lab 

Madi Taylor, Will Kelly, Jacob Green


Question:

What goes through the bag?

 

Procedure:

1) FIll the bag with one of three solutions. (starch, glucose, protein)

2) Weigh the bag and record the weight.

3) Place the bag in a beaker of distilled water.  

4) Repeat for other solutions. 

5) Leave for at least 24 hours.

6) Weigh each bag again and record the weight.

7) Test the distilled water in each beaker for the solution that

was in the dyalisis tubing in that beaker and record the results.

 

Weights:

 

Substance Starting Weight Ending Weight
Starch 5.36 5.78
Glucose 6.36 7.24
Protein 6.74 7.43

 

End Results:

 

Substance Heavier At End Lighter At End Water Test Results 
Starch Yes No Negative
Glucose Yes No Positive
Protein Yes No Negative

 

 

Tests Used:

Test the Starch Water with the IKI Test

Test the Glucose Water with the Benedict Test

Test the Protein Water with the Biuret Test

 

We know this because in the last lab, two substances tested positive with the Benedict test, and the only double substance was a simple sugar (Glucose).  This means that the IKI test, which also tested positive for one of those two substances, was testing for polymers of sugar (Starch).  Therefore, the Biuret test had to test for Protein.

 

Conclusions:

Because the bag of starch solution was heavier at the end of the lab on Monday, 1/26, we can infer that water from the beaker went ino the bag. Also, since the water from the beaker tested negative for starch, we can infer that no starch came out of the bag.

 

Because the bag of glucose solution was heavier at the end of the lab on Monday, 1/26, we can infer that water from the beaker went into the bag. Also, since the water from the beaker tested positive for glucose, we can infer that some glucose from the bag came out and went into the water from the beaker.

 

Becaues the bag of protein solution was heavier at the end of the lab on Monday, 1/26, we can infer that water from the beaker went into the bag. Also, since the water from the beaker tested negative for protein, we can infer that no protein came out of the bag.

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