| 
  • If you are citizen of an European Union member nation, you may not use this service unless you are at least 16 years old.

  • You already know Dokkio is an AI-powered assistant to organize & manage your digital files & messages. Very soon, Dokkio will support Outlook as well as One Drive. Check it out today!

View
 

Biology I 2010 Per 68 Catalase Lab Design

Page history last edited by Claire Chen 14 years, 2 months ago

What are some of the mistakes you made during your last lab?

 

How can you efficiently use your time to answer the question posed?

 

How many times must you do each trial in order to be able to present it to me?

 

You dont actually have to answer these questions.  These are just here to help you plan for the future.

 

This is what Lexi was trying to explain about how to change the pH of something. Like I said before, if you can't read or don't understand something, just tell me and I'll do my best to explain it. (This is Claire, by the way....)

 

Temporary Jobs for the Stuff Before the Lab Begins 

Setting up the pH scale - James, Jacks (both of you), Claire, Gensch (We need to do 11 levels of the pH scale, so thats about two for each of us, and three for one of us)

Making the potato slushie - Cam K. and Alex (sorry if either of you had to do it before)

Ice/Snow Getting - Margaret

Gathering the stuff we need for the lab - Jaime, Lexi, Ryan, Kevin, Drew, Kate

 

I can't believe I actualy broke that down...I'm getting way too panicky over this...

Comments (32)

Cam Gensch said

at 5:18 pm on Feb 16, 2010

We didn't plan out our procedure, where as we should have created set ph's and tested the enzyme's efficiency (time the piece of paper covered in catalase took to float to the top of the water) in each ph, graphing the time it to float on each level of the whole ph scale on a graph.

Cam Gensch said

at 7:40 pm on Feb 16, 2010

just gonna add again.. We could effectively use our time by splitting up into designated groups that have designated jobs

Cam Gensch said

at 10:21 pm on Feb 16, 2010

guess i'm gonna go for the triple threat here cause im not gonna be in class tm.. I'd say three tests per and find the average on each ph test and plot that on the graph

Cam Gensch said

at 10:28 pm on Feb 16, 2010

ah i'm dumb

De said

at 11:42 pm on Feb 16, 2010

You can edit this document. Set up charts. Assign tasks. Just hit the edit button in the top left corner.

Drew Plank said

at 9:25 pm on Feb 17, 2010

Hey guys I literally just got home. I'm in the middle of writing up instructions now. I realized we don't have everything controlled right; we have to make sure the amount of hydrogen peroxide in each beaker is controlled, because if there is more hydrogen peroxide to react with the whole system is thrown off. I know how to fix it (water) but have to figure out the numbers. It might get posted kinda late so I'll make sure I print out copies for everyone.

Drew Plank said

at 9:29 pm on Feb 17, 2010

When I post it it will be in the "Edit" part (where the questions are) instead of the "comment" part

Lexi Van Besien said

at 9:36 pm on Feb 17, 2010

We wasted a lot of time last lab setting up materials. First when we start we should ask for all materials first, then make sure everyone knows their jobs and what they need to do, and last then we begin to mix the acids and bases and test them.

Claire Chen said

at 10:36 pm on Feb 17, 2010

Alright, I've got sort of a "how to" for changing the pH of a substance for those of you who didn't understand/weren't listening/weren't there when we talked about this in the library lab. I'm still trying to figure out how to get a picture on to this page. It's hand-drawn, and due to my terrible handwriting, you might not be able to read it :D. I think I might be able to post pictures in the "edit" part. Just comment if you cant read it and/or don't understand what I'm talking about.

Jack Wollmuth said

at 10:43 pm on Feb 17, 2010

Drew I don't think we are supposed to use water to change the pH...I may be wrong though.

Claire Chen said

at 10:46 pm on Feb 17, 2010

Well, water is the easiest thing to change pH with. While it would be cool and impressive and all that to be able to change it with something else, this is the most straightforward way to change pH.

Hey, should i post a list of materials or something...?

Alex Tung said

at 10:46 pm on Feb 17, 2010

How should we form the groups? Should one person be assigned to test a certain pH level? And should we set a few people to gather the materials in the beginning

Claire Chen said

at 10:53 pm on Feb 17, 2010

@Alex
I'm pretty sure it won't work in pH of 1 or in pH of 14, so we can leave those out. For the rest, we could just set a range and have a few people test each one (it shouldn't take too long, so when someone finishes they could go help other people).
In the beginning, we need people to:
Mash up the potatoes
Get Ice/Snow for the potato slushies
Gather beakers (MAKE SURE THEY'RE GLASS SO WE DONT HAVE CORRODED PLASTIC BEAKERS! O_O)
Those little paper things
Get really strong acid and really strong base
--
Also, someone in each group should have something to time how long it takes for the paper to float up (like ipods, cellphones, watches, etc...)
By the way, refresh the page, I finally got the diagram up XD

Drew Plank said

at 11:00 pm on Feb 17, 2010

The problem with this way is that the newly created pH will have 1/10 the amount of Hydrogen Peroxide;
I have most of my thing done, and I assigned people to do those kinda things

Drew Plank said

at 11:03 pm on Feb 17, 2010

I left that unfinished, oops- if there is less HP, then there will be less reactions, less reactions mean less bubbles, less bubbles mean less rising

Kate Sienko said

at 11:09 pm on Feb 17, 2010

Hi guys, everybody's ideas are great and I think we'll be prepared for tomorrow. Considering we have to present him with a graph of some sort at the end, I will bring my computer to create a graph and I think that somebody else should too since we're splitting into groups

Drew Plank said

at 11:23 pm on Feb 17, 2010

It doesn't seem like anyone is following my logic, but if i exaggerate someone might catch on and help me explain it;
The hydrogen peroxide De gave us was a solution that was 3% hydrogen peroxide and 97% water- we know the potato-mush-covered disk thing rose in that, BUT WHAT IF there is only .0000000000000000000000000000000000000000000000000000000000001% hydrogen peroxide in the solution
That disc is staying on the bottom because there is almost no HP to react with
Please tell me someone gets it because i know it definitely matters

Claire Chen said

at 11:27 pm on Feb 17, 2010

....I dont think it really does matter because the Hydrogen peroxide amount is fixed.

Claire Chen said

at 11:28 pm on Feb 17, 2010

Sorry for double post but what I meant was the concentration of the hydrogen peroxide in what we're using is fixed.

Drew Plank said

at 11:29 pm on Feb 17, 2010

I'll set up the lab- everyone will have asssigned jobs, and I'll make sure the HP level is controlled, because I know it needs to be

Drew Plank said

at 11:33 pm on Feb 17, 2010

Sorry Claire didnt see your post- No its not fixed, because when you take the pH 2 and pour it into water you'll only have a tenth the amount of HP, do that all the way to pH 6 and you'll have a tiny 1/10,000 amount of HP

Claire Chen said

at 11:40 pm on Feb 17, 2010

we should probably set up a person/pair of people to do each pH level (I know we said one group for acid and one group for base before, but i think it'll be easier if everyone concentrates on their own experiment instead of all of us crowding around one.) But have someone set up the pH scale beforehand. (which finally reminds me, we don't need to do any acid or base for pH 6, because that's Hydrogen Peroxide's pH :D)

Claire Chen said

at 11:52 pm on Feb 17, 2010

I think I had a list of preliminary jobs before, but I'll post it again.
We need:
Makers of the potato slushie (1 person or 2 people) (this includes pouring it into the little containers)
A person to gather the ice/snow so the potato slushie doesn't die (yes, only one person)
People to start making the pH's (this will require more people, I'd say 3-4) (MAKE SURE THEY"RE IN GLASS BEAKERS)
I guess in the beginning it'd be nice for some people to gather all the ingredients (the little paper things, the beakers, acid/base, Hydrogen Peroxide, etc.)

Drew Plank said

at 11:53 pm on Feb 17, 2010

Don't ignore the whole "is the Hp equal at all pH's?" thing;
I'll do it like they did it in Math class in like 2nd grade- A glass labeled pH 2 has 90 Black marbles (Acid) and 10 Red marbles (HP) Another glass labeled pH 3 has 90 Blue marbles (Water). If you take 1/10 of the marbles in pH 2 and pour them in the pH 3 what will happen?

9 black marbles and 1 Red Marble will pour into pH 3, leaving it with a final count of 90 Blues, 9 Blacks and 1 Red
Last time I checked 1 did not equal 10, so no its not equal between the pH's- good thing i've been working the last 3 hours finding out how to fix it

Claire Chen said

at 12:01 am on Feb 18, 2010

Please withhold sarcasm until after the lab, thats all I have to say to that.

Would it be alright with the rest of you if I broke us down into groups? Just for the stuff before we actually start the lab, since I'm not exactly sure how we're going to go about actually testing. I'll post it in the edit part, but its only temporary, so if you dont like your job, you can change it with someone else (because I'm not sure what everyone's preference is)

Drew Plank said

at 12:09 am on Feb 18, 2010

How about this for testing- pour 20 ml of HP into every beaker, then I'll use Algebra to figure out how much H20 and Acid/Base to add to make it each HP. Then not only do we have equal HP, but we have mathematically precise pH's. Obviously I'll calculate it before lab not during lol, it'll take forever but its the best way- Then we can just focus on the testing and not worry about the pH being wrong or HP being off

Alex Tung said

at 12:37 am on Feb 18, 2010

we dont have to have the HP in the solution. we just need the final solution to be a certain pH. even though there is .00000000001% of HP in the solution, as long as it is the pH we want it to be it is able to be tested upon.

Claire Chen said

at 12:42 am on Feb 18, 2010

The HP is there so it can be broken down and the paper disk can float to the surface

Drew Plank said

at 12:44 am on Feb 18, 2010

Umm how about I set up pH scale right now? I'll be able to come in with 11 sets of numbers- I can figure it out with Algebra:

X ml of pH 1/13 (Acid/Base) + Y ml of pH 7 (H20) + 20 ml of pH 6.5 (pH of 3% HP solution)= 100ml of pH's 2-12

It's a garanteed control for everything that way, and no guess work involved at all

Drew Plank said

at 1:09 am on Feb 18, 2010

I posted that before cn Alex/Claire's comments sorry- I might have another better explanation of why we need to control the PH concentration;
There are Different Variables that effect the speed of the disc, like W+X+Y+Z
But Mr. De only wants how Z (pH) affects the equation
So we need to make sure that W+X+Y are the same for every beaker, amount of pH is one of those W X Y things

Drew Plank said

at 1:14 am on Feb 18, 2010

They way we have it now the HP consentrantion is different at each pH level, which will screw up results;
My way not only do we control HP, but we also make sure that each pH level is exactly 7 or 6 or, and not 6.5;
I feel like you guys just keep going even though I told you it won't work

Drew Plank said

at 2:57 am on Feb 18, 2010

I tweaked the lab instructions, I don't think its worth the bother of posting, I'll just bring in copies
Minor tweaks to the general plan and it works out fine;
Grouping into Acid and Base groups to create to pH's is the easiest, because each pH relies on the one before it
Then we can split into smaller groups to do the actual time tests

You don't have permission to comment on this page.